Coding

Part:BBa_K932001:Design

Designed by: Sean Kearney   Group: iGEM12_Purdue   (2012-10-03)


EnvZ Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The intention is for production of this protein to amplify expression of the curli cluster. Detailed design required consideration of the interactions of downstream proteins of EnvZ (its regulation of OmpR and subsequent modulation of a number of downstream transcriptional activities). This protein acts in a single-input module network motif.


Source

E. coli K-12 strain MG1655

References